Engineering EGFP reporter constructs into a 200 kb human β-globin BAC clone using GET Recombination

GET Recombination, a simple inducible homologous recombination system for Escherichia coli, was used to target insertion of an EGFP cassette between the start and termination codons of the β-globin gene in a 200 kb BAC clone. The high degree of homology between the promoter regions of the β- and δ-g...

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Vydáno v:	Nucleic Acids Res
Hlavní autoři:	Orford, Michael, Nefedov, Mikhail, Vadolas, Jim, Zaibak, Faten, Williamson, Robert, Ioannou, Panayiotis A.
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Oxford University Press 2000
Témata:	NAR Methods Online
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC110756/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10982895/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.18.e84
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