Investigating the coenzyme specificity of phenylacetone monooxygenase from Thermobifida fusca

Type I Baeyer–Villiger monooxygenases (BVMOs) strongly prefer NADPH over NADH as an electron donor. In order to elucidate the molecular basis for this coenzyme specificity, we have performed a site-directed mutagenesis study on phenylacetone monooxygenase (PAMO) from Thermobifida fusca. Using sequen...

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Detalhes bibliográficos
Main Authors: Dudek, Hanna M., Torres Pazmiño, Daniel E., Rodríguez, Cristina, de Gonzalo, Gonzalo, Gotor, Vicente, Fraaije, Marco W.
Formato: Artigo
Idioma:Inglês
Publicado em: Springer-Verlag 2010
Assuntos:
Biotechnologically Relevant Enzymes and Proteins
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2956058/
https://ncbi.nlm.nih.gov/pubmed/20703875
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00253-010-2769-y
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