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Mass Estimation of Native Proteins by Blue Native Electrophoresis: PRINCIPLES AND PRACTICAL HINTS
Blue native electrophoresis is one of the most popular techniques for mass estimation of native membrane proteins, but the use of non-optimal mass markers and acrylamide gels can compromise accuracy and reliability of the results. We present short protocols taking 10–30 min to prepare optimal sets o...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The American Society for Biochemistry and Molecular Biology
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2953912/ https://ncbi.nlm.nih.gov/pubmed/20173216 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M900526-MCP200 |
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