Functional Characterization of Reductive Dehalogenases by Using Blue Native Polyacrylamide Gel Electrophoresis

Dehalococcoides mccartyi strains are obligate organohalide-respiring bacteria harboring multiple distinct reductive dehalogenase (RDase) genes within their genomes. A major challenge is to identify substrates for the enzymes encoded by these RDase genes. We demonstrate an approach that involves blue...

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Detalhes bibliográficos
Main Authors: Tang, Shuiquan, Chan, Winnie W. M., Fletcher, Kelly E., Seifert, Jana, Liang, Xiaoming, Löffler, Frank E., Edwards, Elizabeth A., Adrian, Lorenz
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2013
Assuntos:
Enzymology and Protein Engineering
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3568550/
https://ncbi.nlm.nih.gov/pubmed/23204411
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01873-12
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