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Mass Estimation of Native Proteins by Blue Native Electrophoresis: PRINCIPLES AND PRACTICAL HINTS

Blue native electrophoresis is one of the most popular techniques for mass estimation of native membrane proteins, but the use of non-optimal mass markers and acrylamide gels can compromise accuracy and reliability of the results. We present short protocols taking 10–30 min to prepare optimal sets o...

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Bibliografische gegevens
Hoofdauteurs: Wittig, Ilka, Beckhaus, Tobias, Wumaier, Zibiernisha, Karas, Michael, Schägger, Hermann
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: The American Society for Biochemistry and Molecular Biology 2010
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2953912/
https://ncbi.nlm.nih.gov/pubmed/20173216
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M900526-MCP200
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