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Use of bicistronic vectors in combination with flow cytometry to screen for effective small interfering RNA target sequences

The efficacy and specificity of small interfering RNAs (siRNAs) are largely dependent on the siRNA sequence. Since only empirical strategies are currently available for predicting these parameters, simple and accurate methods for evaluating siRNAs are needed. To simplify such experiments, target gen...

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Detaylı Bibliyografya
Asıl Yazarlar: Kamio, Naoka, Hirai, Hideyo, Ashihara, Eishi, Tenen, Daniel G., Maekawa, Taira, Imanishi, Jiro
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2010
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC2948238/
https://ncbi.nlm.nih.gov/pubmed/20152796
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrc.2010.02.033
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