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Use of bicistronic vectors in combination with flow cytometry to screen for effective small interfering RNA target sequences
The efficacy and specificity of small interfering RNAs (siRNAs) are largely dependent on the siRNA sequence. Since only empirical strategies are currently available for predicting these parameters, simple and accurate methods for evaluating siRNAs are needed. To simplify such experiments, target gen...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2948238/ https://ncbi.nlm.nih.gov/pubmed/20152796 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrc.2010.02.033 |
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