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Use of bicistronic vectors in combination with flow cytometry to screen for effective small interfering RNA target sequences

The efficacy and specificity of small interfering RNAs (siRNAs) are largely dependent on the siRNA sequence. Since only empirical strategies are currently available for predicting these parameters, simple and accurate methods for evaluating siRNAs are needed. To simplify such experiments, target gen...

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Bibliografske podrobnosti
Main Authors: Kamio, Naoka, Hirai, Hideyo, Ashihara, Eishi, Tenen, Daniel G., Maekawa, Taira, Imanishi, Jiro
Format: Artigo
Jezik:Inglês
Izdano: 2010
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2948238/
https://ncbi.nlm.nih.gov/pubmed/20152796
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrc.2010.02.033
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