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The in vitro characterization of polyene glycosyltransferases AmphDI and NysDI
The overproduction, purification and in vitro characterization of the polyene glycosyltransferases (GTs) AmphDI and NysDI are reported. Enabled by a novel nucleotidyltransferase mutant (RmlA Q83D) for the chemoenzymatic synthesis of unnatural GDP-sugar donors, in conjunction with polyene GT-catalyze...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2947747/ https://ncbi.nlm.nih.gov/pubmed/18798210 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.200800349 |
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