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Development of a universal glycosyltransferase assay amenable to high-throughput formats
The development of a general 1-Zn(II) NDP sensor assay for rapid evaluation of GT activity is described. The 1-Zn(II) NDP sensor assay offers submicromolar sensitivity, compatibility with both purified enzymes and crude cell extracts, and exquisite selectivity for nucleoside diphosphates over the co...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3354722/ https://ncbi.nlm.nih.gov/pubmed/21741952 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2011.06.016 |
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