Llwytho...

Development of a universal glycosyltransferase assay amenable to high-throughput formats

The development of a general 1-Zn(II) NDP sensor assay for rapid evaluation of GT activity is described. The 1-Zn(II) NDP sensor assay offers submicromolar sensitivity, compatibility with both purified enzymes and crude cell extracts, and exquisite selectivity for nucleoside diphosphates over the co...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Lee, Hyun Soo, Thorson, Jon S.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 2011
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC3354722/
https://ncbi.nlm.nih.gov/pubmed/21741952
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2011.06.016
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