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Comparison of Different Signal Thresholds on Data Dependent Sampling in Orbitrap and LTQ Mass Spectrometry for the Identification of Peptides and Proteins in Complex Mixtures

We evaluate the effect of ion-abundance threshold settings for data dependent acquisition on a hybrid LTQ-Orbitrap mass spectrometer, analyzing features such as the total number of spectra collected, the signal to noise ratio of the full MS scans, the spectral quality of the tandem mass spectra acqu...

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Detalhes bibliográficos
Main Authors: Wong, Catherine C.L., Cociorva, Daniel, Venable, John D., Xu, Tao, Yates, John R.
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2946190/
https://ncbi.nlm.nih.gov/pubmed/19467883
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jasms.2009.04.007
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