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Identification of N-terminally arginylated proteins and peptides by mass spectrometry

This protocol describes the essential steps of mass spectrometry-based analysis of protein samples that can be used to identify post-translational arginylation. We describe special considerations for sample preparation and digestion, mass spectrometry analysis using high precision instruments, datab...

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Autors principals: Xu, Tao, Wong, Catherine C.L., Kashina, Anna, Yates, John R.
Format: Artigo
Idioma:Inglês
Publicat: 2009
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2683362/
https://ncbi.nlm.nih.gov/pubmed/19229197
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2008.248
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