Online coupling of reverse-phase and hydrophilic interaction liquid chromatography for protein and glycoprotein characterization

We have developed a novel system for coupling reverse-phase (RP) and hydrophilic interaction liquid chromatography (HILIC) online in a micro-flow scheme. In this approach, the inherent solvent incompatibility between RP and HILIC is overcome through the use of constant-pressure online solvent mixing...

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Detalles Bibliográficos
Main Authors: Lam, Maggie P. Y., Siu, S. O., Lau, Edward, Mao, Xiuli, Sun, H. Z., Chiu, Philip C. N., Yeung, William S. B., Cox, David M., Chu, Ivan K.
Formato: Artigo
Idioma:Inglês
Publicado: Springer-Verlag 2010
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Original Paper
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2939347/
https://ncbi.nlm.nih.gov/pubmed/20632160
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00216-010-3991-2
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