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Protein-induced Conformational Change in Glycans Decreases Resolution of Glycoproteins in Hydrophilic Interaction Liquid Chromatography

An understanding of why hydrophilic interaction liquid chromatography (HILIC) gives higher resolution for glycans than for glycoproteins would facilitate column improvements. Separations of the glycoforms of ribonuclease B compared to its released glycans were studied using a commercial HILIC column...

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Publicat a:J Sep Sci
Autors principals: Bupp, Charles R., Schwartz, Cameron, Wei, Bingchuan, Wirth, Mary J.
Format: Artigo
Idioma:Inglês
Publicat: 2021
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC8076065/
https://ncbi.nlm.nih.gov/pubmed/33682335
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jssc.202001242
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