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Protein-induced Conformational Change in Glycans Decreases Resolution of Glycoproteins in Hydrophilic Interaction Liquid Chromatography

An understanding of why hydrophilic interaction liquid chromatography (HILIC) gives higher resolution for glycans than for glycoproteins would facilitate column improvements. Separations of the glycoforms of ribonuclease B compared to its released glycans were studied using a commercial HILIC column...

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Detalhes bibliográficos
Publicado no:J Sep Sci
Main Authors: Bupp, Charles R., Schwartz, Cameron, Wei, Bingchuan, Wirth, Mary J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8076065/
https://ncbi.nlm.nih.gov/pubmed/33682335
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jssc.202001242
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