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Protein-induced Conformational Change in Glycans Decreases Resolution of Glycoproteins in Hydrophilic Interaction Liquid Chromatography

An understanding of why hydrophilic interaction liquid chromatography (HILIC) gives higher resolution for glycans than for glycoproteins would facilitate column improvements. Separations of the glycoforms of ribonuclease B compared to its released glycans were studied using a commercial HILIC column...

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Bibliographische Detailangaben
Veröffentlicht in:J Sep Sci
Hauptverfasser: Bupp, Charles R., Schwartz, Cameron, Wei, Bingchuan, Wirth, Mary J.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2021
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8076065/
https://ncbi.nlm.nih.gov/pubmed/33682335
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jssc.202001242
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