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Relative quantitation of transfer RNAs using liquid chromatography mass spectrometry and signature digestion products

Transfer ribonucleic acids (tRNAs) are challenging to identify and quantify from unseparated mixtures. Our lab previously developed the signature digestion approach for identifying tRNAs without specific separation. Here we describe the combination of relative quantification via enzyme-mediated isot...

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Autors principals: Castleberry, Colette M., Limbach, Patrick A.
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2010
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2938229/
https://ncbi.nlm.nih.gov/pubmed/20587503
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq578
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