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Sequence mapping of transfer RNA chemical modifications by liquid chromatography tandem mass spectrometry

Mass spectrometry is a powerful analytical tool for identifying and characterizing structural modifications to the four canonical bases in RNA, information that is lost when using techniques such as PCR for RNA analysis. Here we described an updated method for sequence mapping of modified nucleoside...

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Detalhes bibliográficos
Publicado no:Methods
Main Authors: Ross, Robert, Cao, Xiaoyu, Yu, Ningxi, Limbach, Patrick A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5014671/
https://ncbi.nlm.nih.gov/pubmed/27033178
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2016.03.016
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