Detection of All Species of the Genus Alphavirus by Reverse Transcription-PCR with Diagnostic Sensitivity

Clinical arbovirus screening requires exclusion of a broad range of viruses with as few assays as possible. We present a reverse transcription-PCR (RT-PCR) for the detection of all species of the genus Alphavirus qualified for exclusion screening (limit of detection [LOD], 5 to 100 RNA copies per re...

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Detalhes bibliográficos
Main Authors: Grywna, Klaus, Kupfer, Bernd, Panning, Marcus, Drexler, Jan Felix, Emmerich, Petra, Drosten, Christian, Kümmerer, Beate M.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2010
Assuntos:
Virology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2937745/
https://ncbi.nlm.nih.gov/pubmed/20504990
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00317-10
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