Evaluation of Advanced Reverse Transcription-PCR Assays and an Alternative PCR Target Region for Detection of Severe Acute Respiratory Syndrome-Associated Coronavirus

First-generation reverse transcription-PCR (RT-PCR) assays for severe acute respiratory syndrome-associated coronavirus (SARS-CoV) gave false-negative results in a considerable fraction of patients. In the present study, we evaluated two second-generation, replicase (R) gene-based, real-time RT-PCR...

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Pubblicato in:	J Clin Microbiol
Autori principali:	Drosten, Christian, Chiu, Lily-Lily, Panning, Marcus, Leong, Hoe Nam, Preiser, Wolfgang, Tam, John S., Günther, Stephan, Kramme, Stefanie, Emmerich, Petra, Ng, Wooi Loon, Schmitz, Herbert, Koay, Evelyn S. C.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 2004
Soggetti:	Virology
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC404639/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15131168/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.42.5.2043-2047.2004
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