Evaluation of Advanced Reverse Transcription-PCR Assays and an Alternative PCR Target Region for Detection of Severe Acute Respiratory Syndrome-Associated Coronavirus

First-generation reverse transcription-PCR (RT-PCR) assays for severe acute respiratory syndrome-associated coronavirus (SARS-CoV) gave false-negative results in a considerable fraction of patients. In the present study, we evaluated two second-generation, replicase (R) gene-based, real-time RT-PCR...

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Autors principals: Drosten, Christian, Chiu, Lily-Lily, Panning, Marcus, Leong, Hoe Nam, Preiser, Wolfgang, Tam, John S., Günther, Stephan, Kramme, Stefanie, Emmerich, Petra, Ng, Wooi Loon, Schmitz, Herbert, Koay, Evelyn S. C.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2004
Matèries:
Virology
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC404639/
https://ncbi.nlm.nih.gov/pubmed/15131168
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.42.5.2043-2047.2004
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