Generic Detection of Coronaviruses and Differentiation at the Prototype Strain Level by Reverse Transcription-PCR and Nonfluorescent Low-Density Microarray

A nonfluorescent low-cost, low-density oligonucleotide array was designed for detecting the whole coronavirus genus after reverse transcription (RT)-PCR. The limit of detection was 15.7 copies/reaction. The clinical detection limit in patients with severe acute respiratory syndrome was 100 copies/sa...

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Détails bibliographiques
Auteurs principaux: de Souza Luna, Luciano Kleber, Heiser, Volker, Regamey, Nicolas, Panning, Marcus, Drexler, Jan Felix, Mulangu, Sabue, Poon, Leo, Baumgarte, Sigrid, Haijema, Bert Jan, Kaiser, Laurent, Drosten, Christian
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology 2007
Sujets:
Virology
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC1829107/
https://ncbi.nlm.nih.gov/pubmed/17229859
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.02426-06
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