Mammalian cell expression, purification, crystallization and microcrystal data collection of autotaxin/ENPP2, a secreted mammalian glycoprotein

Autotaxin (ATX or ENPP2) is a secreted glycosylated mammalian enzyme that exhibits lysophospholipase D activity, hydrolyzing lysophosphatidylcholine to the signalling lipid lysophosphatidic acid. ATX is an ∼100 kDa multi-domain protein encompassing two N-terminal somatomedin B-like domains, a centra...

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Detalhes bibliográficos
Main Authors: Hausmann, Jens, Christodoulou, Evangelos, Kasiem, Mobien, De Marco, Valeria, van Meeteren, Laurens A., Moolenaar, Wouter H., Axford, Danny, Owen, Robin L., Evans, Gwyndaf, Perrakis, Anastassis
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2010
Assuntos:
Crystallization Communications
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2935246/
https://ncbi.nlm.nih.gov/pubmed/20823545
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110032938
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