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Relative Affinity of Calcium Pump Isoforms for Phospholamban Quantified by Fluorescence Resonance Energy Transfer
To investigate regulation of SERCA1a and SERCA2a calcium pump isoforms by phospholamban (PLB), the proteins were fused to fluorescent protein tags and their interactions were quantified by fluorescence resonance energy transfer (FRET) in live cells. For both SERCA1a or SERCA2a, FRET to PLB increased...
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| Asıl Yazarlar: | , |
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| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
2010
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| Konular: | |
| Online Erişim: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2935190/ https://ncbi.nlm.nih.gov/pubmed/20643144 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2010.07.023 |
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