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Relative Affinity of Calcium Pump Isoforms for Phospholamban Quantified by Fluorescence Resonance Energy Transfer

To investigate regulation of SERCA1a and SERCA2a calcium pump isoforms by phospholamban (PLB), the proteins were fused to fluorescent protein tags and their interactions were quantified by fluorescence resonance energy transfer (FRET) in live cells. For both SERCA1a or SERCA2a, FRET to PLB increased...

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Detalhes bibliográficos
Main Authors: Hou, Zhanjia, Robia, Seth L.
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2935190/
https://ncbi.nlm.nih.gov/pubmed/20643144
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2010.07.023
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