Optimized Nile Red Efflux Assay of AcrAB-TolC Multidrug Efflux System Shows Competition between Substrates

AcrAB-TolC is the major constitutively expressed efflux pump system that provides resistance to a variety of antimicrobial agents and dyes in Escherichia coli. However, no systematically optimized real-time dye efflux assay has been published for the measurement of its activity and for detection of...

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Bibliographic Details
Main Authors: Bohnert, Jürgen A., Karamian, Brian, Nikaido, Hiroshi
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 2010
Subjects:
Mechanisms of Resistance
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2934970/
https://ncbi.nlm.nih.gov/pubmed/20606071
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.00620-10
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