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Structure of cytochrome P450 PimD suggests epoxidation of the polyene macrolide pimaricin occurs via a hydroperoxoferric intermediate

We present the x-ray structure of PimD, both substrate-free and in complex with 4,5-desepoxypimaricin. PimD is a cytochrome P450 monooxygenase with native epoxidase activity that is critical in the biosynthesis of the polyene macrolide antibiotic pimaricin. Intervention in this secondary metabolic p...

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Main Authors: Kells, Petrea M., Ouellet, Hugues, Santos-Aberturas, Javier, Aparicio, Jesus F., Podust, Larissa M.
Formato: Artigo
Idioma:Inglês
Publicado: 2010
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2932657/
https://ncbi.nlm.nih.gov/pubmed/20797613
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chembiol.2010.05.026
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