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Site-Specific Orthogonal Labeling of the Carboxy Terminus of α-Tubulin
A fluorescent probe has been attached to the carboxy terminus of the α-subunit of α,β-tubulin by an enzymatic reaction followed by a chemical reaction. The unnatural amino acid 3-formyltyrosine is attached to the carboxy terminus of α-tubulin through the use of the enzyme tubulin tyrosine ligase. Th...
Tallennettuna:
| Päätekijät: | , , , , , , |
|---|---|
| Aineistotyyppi: | Artigo |
| Kieli: | Inglês |
| Julkaistu: |
2010
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| Aiheet: | |
| Linkit: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2924941/ https://ncbi.nlm.nih.gov/pubmed/20545322 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/cb100060v |
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