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Site-Specific Orthogonal Labeling of the Carboxy Terminus of α-Tubulin

A fluorescent probe has been attached to the carboxy terminus of the α-subunit of α,β-tubulin by an enzymatic reaction followed by a chemical reaction. The unnatural amino acid 3-formyltyrosine is attached to the carboxy terminus of α-tubulin through the use of the enzyme tubulin tyrosine ligase. Th...

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Bibliografski detalji
Glavni autori: Banerjee, Abhijit, Panosian, Timothy D., Mukherjee, Kamalika, Ravindra, Rudravajhala, Gal, Susannah, Sackett, Dan L., Bane, Susan
Format: Artigo
Jezik:Inglês
Izdano: 2010
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2924941/
https://ncbi.nlm.nih.gov/pubmed/20545322
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/cb100060v
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