Native capillary isoelectric focusing for the separation of protein complex isoforms and subcomplexes

Here we report the use of capillary isoelectric focusing under native conditions for the separation of protein complex isoforms and subcomplexes. Using biologically relevant HIS-tag and FLAG-tag purified protein complexes, we demonstrate the separations of protein complex isoforms of the mammalian t...

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Bibliografiset tiedot
Päätekijät: Fonslow, Bryan R., Kang, Seong A., Gestaut, Daniel R., Graczyk, Beth, Davis, Trisha N., Sabatini, David M., Yates, John R.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 2010
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Article
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC2920374/
https://ncbi.nlm.nih.gov/pubmed/20614870
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac101235k
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