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Northern blot detection of endogenous small RNAs (∼14 nt) in bacterial total RNA extracts

Here we describe a northern blot procedure that allows the detection of endogenous RNAs as small as ∼14 nt in total RNA extracts from bacteria. RNAs that small and as part of total bacterial RNA extracts usually escape detection by northern blotting. The approach combines LNA probes 5′-digoxigenin-e...

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Bibliografiset tiedot
Päätekijät: Beckmann, Benedikt M., Grünweller, Arnold, Weber, Michael H. W., Hartmann, Roland K.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Oxford University Press 2010
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC2919735/
https://ncbi.nlm.nih.gov/pubmed/20504856
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq437
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