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Quantitative analysis of endocytosis with cytoplasmic pHluorin chimeras
The pH-sensitive GFP variant pHluorin is typically fused to the extracellular domain of transmembrane proteins to monitor endocytosis. Here we have turned pHluorin inside-out, and demonstrate that cytoplasmic fusions of pHluorin are effective quantitative reporters for endocytosis and MVB sorting. I...
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| Autors principals: | , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2010
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2919640/ https://ncbi.nlm.nih.gov/pubmed/20626707 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1600-0854.2010.01088.x |
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