Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast

Green fluorescent protein (GFP) and its variants are widely used tools for studying protein localization and dynamics of events such as cytoskeletal remodeling and vesicular trafficking in living cells. Quantitative methodologies using chimeric GFP fusions have been developed for many applications;...

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Bibliografiska uppgifter
I publikationen:J Vis Exp
Huvudupphovsmän: Prosser, Derek C., Wrasman, Kristie, Woodard, Thaddeus K., O’Donnell, Allyson F., Wendland, Beverly
Materialtyp: Artigo
Språk:Inglês
Publicerad: MyJove Corporation 2016
Ämnen:
Molecular Biology
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC5092240/
https://ncbi.nlm.nih.gov/pubmed/27805610
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54587
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