Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast

Green fluorescent protein (GFP) and its variants are widely used tools for studying protein localization and dynamics of events such as cytoskeletal remodeling and vesicular trafficking in living cells. Quantitative methodologies using chimeric GFP fusions have been developed for many applications;...

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Bibliografski detalji
Izdano u:J Vis Exp
Glavni autori: Prosser, Derek C., Wrasman, Kristie, Woodard, Thaddeus K., O’Donnell, Allyson F., Wendland, Beverly
Format: Artigo
Jezik:Inglês
Izdano: MyJove Corporation 2016
Teme:
Molecular Biology
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5092240/
https://ncbi.nlm.nih.gov/pubmed/27805610
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54587
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