Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast

Green fluorescent protein (GFP) and its variants are widely used tools for studying protein localization and dynamics of events such as cytoskeletal remodeling and vesicular trafficking in living cells. Quantitative methodologies using chimeric GFP fusions have been developed for many applications;...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:J Vis Exp
Prif Awduron: Prosser, Derek C., Wrasman, Kristie, Woodard, Thaddeus K., O’Donnell, Allyson F., Wendland, Beverly
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: MyJove Corporation 2016
Pynciau:
Molecular Biology
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC5092240/
https://ncbi.nlm.nih.gov/pubmed/27805610
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54587
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