Llwytho...

Quantitative analysis of endocytosis with cytoplasmic pHluorin chimeras

The pH-sensitive GFP variant pHluorin is typically fused to the extracellular domain of transmembrane proteins to monitor endocytosis. Here we have turned pHluorin inside-out, and demonstrate that cytoplasmic fusions of pHluorin are effective quantitative reporters for endocytosis and MVB sorting. I...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Prosser, Derek C., Whitworth, Karen, Wendland, Beverly
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 2010
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC2919640/
https://ncbi.nlm.nih.gov/pubmed/20626707
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1600-0854.2010.01088.x
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