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Method for Designing and Optimizing Random-Search Libraries for Strain Improvement

Random searches have been the hallmark of directed evolution and have been extensively employed in the improvement of complex or poorly understood phenotypes such as tolerance to toxic compounds in the context of cellular engineering. While genome-wide mutagenesis followed by selection or screening...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Klein-Marcuschamer, Daniel, Stephanopoulos, Gregory
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology (ASM) 2010
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2918980/
https://ncbi.nlm.nih.gov/pubmed/20581192
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00828-10
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