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Method for Designing and Optimizing Random-Search Libraries for Strain Improvement

Random searches have been the hallmark of directed evolution and have been extensively employed in the improvement of complex or poorly understood phenotypes such as tolerance to toxic compounds in the context of cellular engineering. While genome-wide mutagenesis followed by selection or screening...

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Detalhes bibliográficos
Main Authors: Klein-Marcuschamer, Daniel, Stephanopoulos, Gregory
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2918980/
https://ncbi.nlm.nih.gov/pubmed/20581192
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00828-10
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