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SDS-PAGE-MALDI-TDS: Mass Spectrometric Top-Down Sequencing of Proteins Extracted from Polyacrylamide Gels

RP-86 MALDI top-down sequencing (TDS) using In-Source-Decay (ISD) is a powerful tool to analyze protein termini without prior tryptic digestion. Sequence calls up to 80 residues of each terminus can be achieved. In addition, the technique is well suited to detect modifications, truncations, and muta...

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Bibliografski detalji
Glavni autori: Evers, W., Resemann, A., Irrgang, J., Dibowski, H., Razunguzwa, T.T., Suckau, D.
Format: Artigo
Jezik:Inglês
Izdano: Association of Biomolecular Resource Facilities 2010
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2918097/
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