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SDS-PAGE-MALDI-TDS: Mass Spectrometric Top-Down Sequencing of Proteins Extracted from Polyacrylamide Gels

RP-86 MALDI top-down sequencing (TDS) using In-Source-Decay (ISD) is a powerful tool to analyze protein termini without prior tryptic digestion. Sequence calls up to 80 residues of each terminus can be achieved. In addition, the technique is well suited to detect modifications, truncations, and muta...

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Main Authors: Evers, W., Resemann, A., Irrgang, J., Dibowski, H., Razunguzwa, T.T., Suckau, D.
格式: Artigo
語言:Inglês
出版: Association of Biomolecular Resource Facilities 2010
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC2918097/
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