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Expression, purification, crystallization and preliminary X-ray studies of Lactobacillus jensenii enolase
Recombinant Lactobacillus jensenii enolase fused to a C-terminal noncleavable His tag was expressed in Escherichia coli, purified and crystallized by sitting-drop vapor diffusion. A complete data set was collected to 3.25 Å resolution. The crystals belonged to space group I4, with unit-cell paramete...
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| Autores principales: | , , , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
International Union of Crystallography
2010
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2917297/ https://ncbi.nlm.nih.gov/pubmed/20693674 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110022748 |
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