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Purification, crystallization and preliminary X-ray diffraction analysis of human enolase-phosphatase E1

Enolase-phosphatase E1 (MASA) is a bifunctional enzyme in the ubiquitous methionine-salvage pathway and catalyzes the continuous reaction of 2,3-­diketo-5-methylthio-1-phosphopentane to yield the acireductone metabolite. Recombinant human E1 enzyme has been crystallized using the hanging-drop vapour...

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Main Authors: Wang, Hui, Pang, Hai, Ding, Yi, Li, Yi, Wu, Xiao’ai, Rao, Zihe
Formato: Artigo
Idioma:Inglês
Publicado: International Union of Crystallography 2005
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC1952300/
https://ncbi.nlm.nih.gov/pubmed/16511085
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430910501184X
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