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Overexpression, purification, crystallization and preliminary X-ray studies of Vibrio cholerae EpsG

EpsG is the major pseudopilin protein of the Vibrio cholerae type II secretion system. An expression plasmid that encodes an N-terminally truncated form of EpsG with a C-terminal noncleavable His tag was constructed. Recombinant EpsG was expressed in Escherichia coli; the truncated protein was purif...

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Detalhes bibliográficos
Main Authors: Jens, Jason, Raghunathan, Kannan, Vago, Frank, Arvidson, Dennis
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2688428/
https://ncbi.nlm.nih.gov/pubmed/19478449
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309109017163
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