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Overexpression, purification, crystallization and preliminary X-ray studies of Vibrio cholerae EpsG
EpsG is the major pseudopilin protein of the Vibrio cholerae type II secretion system. An expression plasmid that encodes an N-terminally truncated form of EpsG with a C-terminal noncleavable His tag was constructed. Recombinant EpsG was expressed in Escherichia coli; the truncated protein was purif...
Tallennettuna:
| Päätekijät: | , , , |
|---|---|
| Aineistotyyppi: | Artigo |
| Kieli: | Inglês |
| Julkaistu: |
International Union of Crystallography
2009
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| Aiheet: | |
| Linkit: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2688428/ https://ncbi.nlm.nih.gov/pubmed/19478449 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309109017163 |
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