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Target Immunity of the Tn3-Family Transposon Tn4430 Requires Specific Interactions between the Transposase and the Terminal Inverted Repeats of the Transposon

Specificity of the Tn4430 target immunity signal was examined by fusing the transposase TnpA to the LacI repressor of Escherichia coli. The resulting chimeric proteins failed to impose immunity to DNA targets carrying copies of the lacO operator, though they were proficient in lacO binding in vivo a...

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Detalhes bibliográficos
Main Authors: Nicolas, Emilien, Lambin, Michaël, Hallet, Bernard
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2916420/
https://ncbi.nlm.nih.gov/pubmed/20562304
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.00477-10
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