QUANTITATION OF PI[3,4,5]P3 DYNAMICS IN EGF-STIMULATED CARCINOMA CELLS: A comparison of PH domain-mediated versus immunological methods

Class IA phosphoinositide 3-kinases (PI3-kinase) generate the secondary messenger PI[3,4,5]P3, which plays an important role in many cellular responses. The accumulation of PI[3,4,5]P3 in cell membranes is routinely measured using GFP-labeled PH domains. However, the kinetics of membrane PI[3,4,5]P3...

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Detalhes bibliográficos
Main Authors: Yip, Shu-Chin, Eddy, Robert J., Branch, Angie M., Pang, Huan, Wu, Haiyan, Yan, Ying, Drees, Beth E., Neilsen, Paul O., Condeelis, John, Backer, Jonathan M.
Formato: Artigo
Idioma:Inglês
Publicado em: 2008
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2915465/
https://ncbi.nlm.nih.gov/pubmed/18215145
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20071179
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