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Combinatorial Method for Overexpression of Membrane Proteins in Escherichia coli
Membrane proteins constitute 20–30% of all proteins encoded by the genome of various organisms. Large amounts of purified proteins are required for activity and crystallization attempts. Thus, there is an unmet need for a heterologous membrane protein overexpression system for purification, crystall...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Biochemistry and Molecular Biology
2010
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2911344/ https://ncbi.nlm.nih.gov/pubmed/20525689 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M110.125492 |
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