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Combinatorial Method for Overexpression of Membrane Proteins in Escherichia coli

Membrane proteins constitute 20–30% of all proteins encoded by the genome of various organisms. Large amounts of purified proteins are required for activity and crystallization attempts. Thus, there is an unmet need for a heterologous membrane protein overexpression system for purification, crystall...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Leviatan, Shani, Sawada, Keisuke, Moriyama, Yoshinori, Nelson, Nathan
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: American Society for Biochemistry and Molecular Biology 2010
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC2911344/
https://ncbi.nlm.nih.gov/pubmed/20525689
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M110.125492
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