Increasing the sensitivity of reverse phase protein arrays by antibody-mediated signal amplification

BACKGROUND: Reverse phase protein arrays (RPPA) emerged as a useful experimental platform to analyze biological samples in a high-throughput format. Different signal detection methods have been described to generate a quantitative readout on RPPA including the use of fluorescently labeled antibodies...

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Main Authors: Brase, Jan C, Mannsperger, Heiko, Fröhlich, Holger, Gade, Stephan, Schmidt, Christian, Wiemann, Stefan, Beissbarth, Tim, Schlomm, Thorsten, Sültmann, Holger, Korf, Ulrike
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2010
Fag:
Methodology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2908584/
https://ncbi.nlm.nih.gov/pubmed/20569466
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1477-5956-8-36
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