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RNAi-based validation of antibodies for reverse phase protein arrays

BACKGROUND: Reverse phase protein arrays (RPPA) have been demonstrated to be a useful experimental platform for quantitative protein profiling in a high-throughput format. Target protein detection relies on the readout obtained from a single detection antibody. For this reason, antibody specificity...

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Autors principals: Mannsperger, Heiko A, Uhlmann, Stefan, Schmidt, Christian, Wiemann, Stefan, Sahin, Özgür, Korf, Ulrike
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2010
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3022873/
https://ncbi.nlm.nih.gov/pubmed/21182776
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1477-5956-8-69
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