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A Novel Kinetic Assay of Mitochondrial ATP-ADP Exchange Rate Mediated by the ANT
A novel method exploiting the differential affinity of ADP and ATP to Mg(2+) was developed to measure mitochondrial ADP-ATP exchange rate. The rate of ATP appearing in the medium after addition of ADP to energized mitochondria, is calculated from the measured rate of change in free extramitochondria...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
The Biophysical Society
2009
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2907717/ https://ncbi.nlm.nih.gov/pubmed/19289073 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2008.12.3915 |
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