Mitigating Unwanted Photophysical Processes for Improved Single-Molecule Fluorescence Imaging

Organic fluorophores common to fluorescence-based investigations suffer from unwanted photophysical properties, including blinking and photobleaching, which limit their overall experimental performance. Methods to control such processes are particularly important for single-molecule fluorescence and...

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Bibliographic Details
Main Authors: Dave, Richa, Terry, Daniel S., Munro, James B., Blanchard, Scott C.
Format: Artigo
Language:Inglês
Published: The Biophysical Society 2009
Subjects:
Spectroscopy, Imaging, Other Techniques
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2907709/
https://ncbi.nlm.nih.gov/pubmed/19289062
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2008.11.061
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