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Room Temperature Spectrally Resolved Single-Molecule Spectroscopy Reveals New Spectral Forms and Photophysical Versatility of Aequorea Green Fluorescent Protein Variants
It is known from ensemble spectroscopy at cryogenic temperatures that variants of the Aequorea green fluorescent protein (GFP) occur in interconvertible spectroscopically distinct forms which are obscured in ensemble room temperature spectroscopy. By analyzing the fluorescence of the GFP variants EY...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Biophysical Society
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1304926/ https://ncbi.nlm.nih.gov/pubmed/15454402 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.104.049452 |
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